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ATCC
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Becton Dickinson
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Becton Dickinson
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Revvity
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Biogems International
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Image Search Results
Journal: The EMBO Journal
Article Title: Notch ligand Dll4 impairs cell recruitment to aortic clusters and limits blood stem cell generation
doi: 10.15252/embj.2019104270
Figure Lengend Snippet: We analyzed the peripheral blood and bone marrow from transplanted irradiated mice after 4 months. CD45.1 or CD45.2 donor or recipient cells were analyzed for the contribution to the main hematopoietic lineages in the mice where engraftment was detected. Mac1 (myeloid lineage), CD3 (T lymphoid lineage), B220 (B lymphoid lineage), and Ter119 (erythroid lineage). Graphs represent the percentage of lineage cells derived from IgG‐treated (n = 4) and aDll4 (n = 7)‐treated AGM.
Article Snippet: Antibody staining was performed in PBS supplemented with 10% FCS in the dark, at room temperature for 15 min, or carried out on ice for 30 min. CD117 (c‐kit; APC‐Cy7 or APC‐eFluor780), CD31 (APC or FITC),
Techniques: Irradiation, Derivative Assay
Journal: The EMBO Journal
Article Title: Pericyte signaling via soluble guanylate cyclase shapes the vascular niche and microenvironment of tumors
doi: 10.1038/s44318-024-00078-5
Figure Lengend Snippet: ( A ) Representative fluorescent images depicting CD31 and NG2-stained tumor sections from sGC ctr and sGC ΔPC mice. Plot showing the percentage of vessels covered by NG2-positive pericytes. Data presented as mean ± SD, with n = 3–4 mice per group. Scale bar, 100 µm. ( B ) Representative fluorescent images displaying DAPI and Ter119-stained LLC tumor sections from sGC ctr and sGC ΔPC mice. Plot showing the Ter119-positive area in tumors. Data presented as mean ± SD, with n = 5 mice per group. Scale bar, 1 mm. ( C ) Representative fluorescent images displaying DAPI, Lectin and CD31-stained LLC tumor sections obtained from sGC ctr and sGC ΔPC mice. Arrows indicate non-perfused vessels. Plot showing the functional vessel area in tumors. Data presented as mean ± SD, with n = 5 mice per group. Scale bar, 300 µm. Statistical significance assessed using two-tailed Student’s t test ( A – C ). * P < 0.05; ** P < 0.01. .
Article Snippet: The following antibodies were used for quantifying pericytes in sGC-EGFP transgenic mice: APC-conjugated anti-CD41 (1:100, 133914, Biolegend),
Techniques: Staining, Functional Assay, Two Tailed Test